{"id":1836,"date":"2024-04-28T17:04:04","date_gmt":"2024-04-28T09:04:04","guid":{"rendered":"https:\/\/www.yearnintl.com\/?p=1836"},"modified":"2024-04-28T17:04:04","modified_gmt":"2024-04-28T09:04:04","slug":"application-of-dimethyl-sulfoxide-in-cell-cryopreservation","status":"publish","type":"post","link":"https:\/\/www.yearnintl.com\/ko\/problems\/application-of-dimethyl-sulfoxide-in-cell-cryopreservation\/","title":{"rendered":"\uc138\ud3ec \ub0c9\ub3d9 \ubcf4\uc874\uc5d0 \ub514\uba54\ud2f8 \uc124\ud3ed\uc0ac\uc774\ub4dc \uc801\uc6a9"},"content":{"rendered":"<p>\ub514\uba54\ud2f8\uc124\ud3ed\uc0ac\uc774\ub4dc\ub294 \uc911\uc694\ud55c \uc0bc\ud22c\uc131 \uc138\ud3ec \ubcf4\ud638\uc81c\uc785\ub2c8\ub2e4. \uc2ec\ubd80\uc628\ub3c4(\uc601\ud558 200\ub3c4)\uc5d0\uc11c \uc138\ud3ec\ub97c \ub3d9\uacb0\ubcf4\uc874\ud558\ub294 \uacfc\uc815\uc5d0\uc11c \uc138\ud3ec\ub0b4 \uc561\uccb4\uc5bc\uc74c\uacb0\uc815\uc758 \ud615\uc131, \uc0bc\ud22c\uc555 \ubcc0\ud654, \uc138\ud3ec\uad6c\uc870 \uc7a5\uc560\ub85c \uc778\ud55c \uc190\uc0c1\uc744 \uc608\ubc29\ud558\uae30 \uc704\ud574\uc11c\ub294 DMSO\uac00 \ud568\uc720\ub41c \ub3d9\uacb0\ubcf4\ud638\uc81c\uc758 \uc0ac\uc6a9\uc774 \ud544\uc694\ud558\ub2e4.<br \/>DMSO\ub294 \uc138\ud3ec\ub9c9\uc744 \uc138\ud3ec \ub0b4\ubd80\ub85c \ube60\ub974\uac8c \uce68\ud22c\ud558\uc5ec \uc5b4\ub294\uc810\uc744 \ub0ae\ucd94\uace0, \uc5b4\ub294 \uacfc\uc815\uc744 \uc9c0\uc5f0\uc2dc\ud0a4\uba70, \uc138\ud3ec\ub0b4 \uc774\uc628 \ub18d\ub3c4\ub97c \ub192\uc774\uace0, \uc138\ud3ec\ub0b4 \uc5bc\uc74c \uacb0\uc815\uc758 \ud615\uc131\uc744 \uac10\uc18c\uc2dc\ucf1c \uc138\ud3ec \uc190\uc0c1\uc744 \uc904\uc77c \uc218 \uc788\uc2b5\ub2c8\ub2e4.<br \/>\u200b<br \/>\ub514\uba54\ud2f8\uc220\ud3ed\uc2dc\ub4dc\uc758 \uc138\ud3ec\ub3c5\uc131\uc740 \ub9e4\uc6b0 \ub0ae\uc740 \uc628\ub3c4\uc5d0\uc11c \uc5b5\uc81c\ub418\uba70 \uc18c\uc0dd \uc2dc \uc791\uc6a9\uc774 \ube68\ub77c\uc11c \uac00\ub2a5\ud55c \ud55c \ube68\ub9ac \ub514\uba54\ud2f8\uc220\ud3ed\uc2dc\ub4dc\ub97c \uc53b\uc5b4\ub0b4\uc57c \ud569\ub2c8\ub2e4. \uadf8\ub807\uc9c0 \uc54a\uc73c\uba74 \uc138\ud3ec\uc5d0 \uc2ec\uac01\ud55c \ub3c5\uc131\uc744 \uc720\ubc1c\ud560 \uc218 \uc788\uc2b5\ub2c8\ub2e4.<br \/>\ub514\uba54\ud2f8\uc124\ud3ed\uc0ac\uc774\ub4dc(DMSO)\ub294 \ub0c9\ub3d9\ubcf4\uc874\ub41c \uc138\ud3ec\uc5d0 \ub300\ud55c \ucd5c\uace0\uc758 \ubcf4\ud638\uc81c\uc774\uc9c0\ub9cc \uc138\ud3ec\ub3c5\uc131\uc774 \ub192\uc740 \ud654\ud559\uc801 \uc2dc\ud5d8\uc81c\uc774\uae30\ub3c4 \ud569\ub2c8\ub2e4. \uacb0\uacfc\ub294 \ubc30\uc591 \ubc30\uc9c0 \ub0b4 DMSO\uc758 \ub18d\ub3c4\uac00 10%\uc77c \ub54c \uc138\ud3ec \uc131\uc7a5 \uc5b5\uc81c\uc728\uc774 \uac70\uc758 100%\uc784\uc744 \ubcf4\uc5ec\uc90d\ub2c8\ub2e4. 1\u2030 \ub18d\ub3c4\uc5d0\uc11c\ub294 \uc5b5\uc81c\uc728\uc774 35%\uc600\uc73c\uba70, 0.04\u2030 \ub18d\ub3c4\uc5d0\uc11c\ub3c4 DMSO\ub294 \uc138\ud3ec \uc131\uc7a5\uc5d0 \ubd80\uc815\uc801\uc778 \uc601\ud5a5\uc744 \ubbf8\ucce4\ub2e4.<br \/><img decoding=\"async\" class=\"alignnone size-full wp-image-1214\" src=\"https:\/\/www.yearnintl.com\/wp-content\/uploads\/2023\/12\/nmp.jpg\" alt=\"dmso\" width=\"379\" height=\"378\" \/><br \/>\uc2e4\ud5d8\uc5d0\uc11c\ub294 \uc5b4\ub5bb\uac8c \uc0ac\uc6a9\ud558\ub098\uc694? \ub2e4\uc74c\uc5d0 \ub9d0\uc500\ub4dc\ub9b4\uac8c\uc694<br \/>01 \uc138\ud3ec \ub0c9\ub3d9\ubcf4\uc874<br \/>\u2460 10%DMSO \ub610\ub294 \uae00\ub9ac\uc138\ub9b0\uacfc 10~20% \uc1a1\uc544\uc9c0\ud608\uccad\uc744 \ud568\uc720\ud55c \ub3d9\uacb0\ubc30\uc591\uc561\uc744 \uc81c\uc870\ud558\uc600\ub2e4.<br \/>\u2461 \ub300\uc218\uc99d\uc2dd\uae30\uc758 \uc138\ud3ec\ub97c \ucc44\ucde8\ud558\uc5ec \uae30\uc874 \ubc30\uc591\uc561\uc744 \uc81c\uac70\ud558\uace0 PBS\ub85c \uc138\ucc99\ud569\ub2c8\ub2e4.<br \/>\u2462 PBS\ub97c \uc81c\uac70\ud558\uace0 \uc801\ub2f9\ub7c9\uc758 \ud2b8\ub9bd\uc2e0\uc744 \ucca8\uac00\ud558\uc5ec(\ud398\ud2b8\ub9ac \uc811\uc2dc \ud45c\uba74\uc744 \ub36e\uc74c) \ub2e8\uc77c\uce35\uc758 \uc131\uc7a5\ud558\ub294 \uc138\ud3ec\ub97c \uc18c\ud654\uc2dc\ucf30\ub2e4.<br \/>\u2463 1000rpm\uc73c\ub85c 5\ubd84\uac04 \uc6d0\uc2ec\ubd84\ub9ac\ud55c\ub2e4.<br \/>\u2464 \ud2b8\ub9bd\uc2e0\uc744 \uc81c\uac70\ud558\uace0 \uc900\ube44\ub41c \ub0c9\ub3d9\ubc30\uc591\uc561 \uc801\ub2f9\ub7c9\uc744 \ucca8\uac00\ud55c \ud6c4 \ube68\ub300\ub85c \uac00\ubccd\uac8c \ubd88\uc5b4 \uc138\ud3ec\ub97c \uade0\uc77c\ud558\uac8c \ud55c \ud6c4 \uacc4\uc218\ud558\uace0 \ucd5c\uc885 \ub0c9\ub3d9\uc561\uc758 \uc138\ud3ec\ubc00\ub3c4\ub97c 5\u00d7106\/ml ~ 1\u00d7107\/ml\ub85c \uc870\uc815\ud55c\ub2e4.<br \/>(6) \uc138\ud3ec\ub97c \ub0c9\ub3d9\ubcf4\uad00\uc6a9 \ud29c\ube0c\ub85c \ub098\ub204\uc5b4 \uac01 \ud29c\ube0c\ub9c8\ub2e4 1~1.5ml\uc529 \ub2f4\uc558\ub2e4.<br \/>\u2466 \ub0c9\ub3d9\ubcf4\uad00\uc6a9\uae30\uc5d0 \uc138\ud3ec\uba85, \ub0c9\ub3d9\uc2dc\uac04, \uc791\uc5c5\uc790\ub97c \uae30\uc7ac\ud569\ub2c8\ub2e4.<br \/>\ub0c9\ub3d9: \ud45c\uc900 \ub0c9\ub3d9 \uc808\ucc28\ub294 -1 ~ -2\u2103\/min\uc758 \ub0c9\uac01 \uc18d\ub3c4\uc785\ub2c8\ub2e4. \uc628\ub3c4\uac00 -25\u2103 \ubbf8\ub9cc\uc5d0 \ub3c4\ub2ec\ud558\uba74 -5\u2103 ~ -10\u2103\/min\uae4c\uc9c0 \uc99d\uac00\ud560 \uc218 \uc788\uc2b5\ub2c8\ub2e4. -100\u00b0C\uc5d0\uc11c\ub294 \uc561\uccb4\uc9c8\uc18c\uc5d0 \ube60\ub974\uac8c \ub2f4\uae00 \uc218 \uc788\uc2b5\ub2c8\ub2e4. \ub610\ub294 \uc138\ud3ec\uac00 \ub4e4\uc5b4 \uc788\ub294 \ub0c9\ub3d9\ubcf4\uad00 \ud29c\ube0c\ub97c -20\u2103 \ub0c9\uc7a5\uace0\uc5d0 2\uc2dc\uac04 \ub3d9\uc548 \ub123\uc740 \ud6c4 -70\u2103 \ub0c9\uc7a5\uace0\uc5d0 \ubc24\uc0c8 \ubcf4\uad00\ud55c \ud6c4 \ub0c9\ub3d9\ubcf4\uad00 \ud29c\ube0c\ub97c \uaebc\ub0b4 \uc561\uccb4\uc9c8\uc18c \uc6a9\uae30\uc5d0 \ub123\uc744 \uc218\ub3c4 \uc788\uc2b5\ub2c8\ub2e4.<br \/>02 \uc138\ud3ec\ud68c\ubcf5<br \/>\u2460 \uc561\uccb4\uc9c8\uc18c \uc6a9\uae30\uc5d0\uc11c \ub0c9\ub3d9\ubcf4\uad00\uad00\uc744 \uaebc\ub0b4\uc5b4 37\u2103 \ubbf8\uc9c0\uadfc\ud55c \ubb3c\uc5d0 \uc9c1\uc811 \ub2f4\uadfc \ud6c4 \uc218\uc2dc\ub85c \ud754\ub4e4\uc5b4 \ub179\uc5ec\uc8fc\uc138\uc694.<br \/>\u2461 37\u2103 \uc218\uc870\uc5d0\uc11c \ub0c9\ub3d9\ubcf4\uc874\ud29c\ube0c\ub97c \uaebc\ub0b4\uc5b4 \ub69c\uaed1\uc744 \uc5f4\uace0 \uc138\ud3ec\ud604\ud0c1\uc561\uc744 \ube68\ub300\ub85c \ud761\uc785\ud55c \ud6c4 \uc6d0\uc2ec\ubd84\ub9ac\ud29c\ube0c\uc5d0 \ub123\uace0 \ubc30\uc591\uc561\uc744 10\ubc30 \uc774\uc0c1 \ucca8\uac00\ud558\uc5ec \uc798 \uc11e\ub294\ub2e4.<br \/>\u2462 \uc6d0\uc2ec\ubd84\ub9ac\uae30, 1000rpm, 5\ubd84<br \/>\u2463 \uc0c1\uce35\uc561\uc744 \ubc84\ub9ac\uace0 10% \uc1a1\uc544\uc9c0\ud608\uccad\uc774 \ud568\uc720\ub41c \ubc30\uc591\ubc30\uc9c0\ub97c \uc7ac\ud604\ud0c1\uc138\ud3ec\uc5d0 \ucca8\uac00\ud558\uc5ec \uacc4\uc218\ud558\uace0 \uc138\ud3ec\ubc00\ub3c4\ub97c \uc870\uc808\ud55c \ud6c4 \ubc30\uc591\ubcd1\uc5d0 \uc811\uc885\ud558\uc5ec 37\u2103\uc5d0\uc11c \ubc30\uc591\ud55c\ub2e4.<br \/>\u2464 \ub2e4\uc74c\ub0a0 \ubc30\uc591\uc561\uc744 \uad50\uccb4\ud558\uace0 \uacc4\uc18d \ubc30\uc591\ud55c\ub2e4.<br \/>\uadf8\ub7fc \uadf8\uac8c DMSO\uc8e0<br \/>\uc138\ud3ec \ub0c9\ub3d9\ubcf4\uc874\uc5d0 \uc0ac\uc6a9\ud558\ub294 \ubc29\ubc95<br \/>DMSO \ubc0f \uae30\ud0c0 \ud654\ud559 \uc6d0\ub8cc, \uc2dc\uc57d \ud310\ub9e4 \uac00\ub2a5<\/p>","protected":false},"excerpt":{"rendered":"<p>Dimethyl sulfoxide is an important osmotic cell protectant. It is necessary to use a cryoprotectant containing DMSO in order to prevent damage caused by the formation of intracellular liquid ice crystals, osmotic pressure changes, and cell structure disorders during the cryopreserved process of cells at a deep temperature (minus 200 degrees Celsius).DMSO can quickly penetrate the cell membrane into the cell, reduce the freezing point, delay the freezing process, increase the concentration of intracellular ions, reduce the formation of intracellular ice crystals, and thus reduce cell damage.\u200bThe cytotoxicity of dimethyl sulfoxide is inhibited at deep low temperature, and the action should be fast during resuscitation to wash off dimethyl sulfoxide as soon as possible, otherwise it will cause serious toxicity to cells.Dimethyl sulfoxide (DMSO) is the best protective agent for cell cryopreserved, but it is also a chemical test agent with high cytotoxicity. The results show that when the concentration\u2026<\/p>","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[3],"tags":[],"attr":[23],"class_list":["post-1836","post","type-post","status-publish","format-standard","hentry","category-problems"],"_links":{"self":[{"href":"https:\/\/www.yearnintl.com\/ko\/wp-json\/wp\/v2\/posts\/1836","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.yearnintl.com\/ko\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.yearnintl.com\/ko\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.yearnintl.com\/ko\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.yearnintl.com\/ko\/wp-json\/wp\/v2\/comments?post=1836"}],"version-history":[{"count":1,"href":"https:\/\/www.yearnintl.com\/ko\/wp-json\/wp\/v2\/posts\/1836\/revisions"}],"predecessor-version":[{"id":1837,"href":"https:\/\/www.yearnintl.com\/ko\/wp-json\/wp\/v2\/posts\/1836\/revisions\/1837"}],"wp:attachment":[{"href":"https:\/\/www.yearnintl.com\/ko\/wp-json\/wp\/v2\/media?parent=1836"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.yearnintl.com\/ko\/wp-json\/wp\/v2\/categories?post=1836"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.yearnintl.com\/ko\/wp-json\/wp\/v2\/tags?post=1836"},{"taxonomy":"attr","embeddable":true,"href":"https:\/\/www.yearnintl.com\/ko\/wp-json\/wp\/v2\/attr?post=1836"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}